Medicinal Plant

2010, v.1(08) 22-25+28

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Study on Rooting Culture of Sarcardra glabra(Thunb.) Nakai tube plantlet
Study on Rooting Culture of Sarcardra glabra(Thunb.) Nakai tube plantlet

摘要(Abstract):

[Objective] To establish an efficient rooting protocol in vitro for perfecting tissue culture system of Sarcandra glabra(Thunb.) Nakai.[Method] The regenerated buds of Sarcandra glabra(Thunb.) Nakai.as tested materials,the effects of different concentrations and types of auxins,inorganic salt and sucrose on adventitious root formation were studied.[Result] NAA went against the rooting induction of Sarcandra glabra(Thunb.) Nakai..IBA could improve the rooting speed and the root system quality,the optimum concentration of which was 0.2 mg/L.Lower mineral strength such as 1/2MS or 1/4MS was much better for rooting of Sarcandra glabra(Thunb.) Nakai..Too high or too low sucrose concentration had some restrained effect on root formation,the best induction results were obtained at the concentration of 30 g/L.[Conclusion] The optimal rooting media of Sarcandra glabra(Thunb.) Nakai.was 1/2MS+IBA0.2 mg/L +sucrose 30 g/L or 1/4 MS+IBA 0.2 mg/L +sucrose 30 g/L.

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基金项目(Foundation): Supported by Huzhou Technological and Scientific Project(2008YN10);; Qianjiang Talented Person Project (2009R10016)

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