Medicinal Plant

2012, v.3(11) 29-31

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Study on the Extraction and Purification Process of Esculetin from CORTEX FRAXINI
Study on the Extraction and Purification Process of Esculetin from CORTEX FRAXINI

YU Ling;LIU Ying;YAN Ting;WEN Meng;

摘要(Abstract):

[Objective] To study the optimal extraction and purification process of esculetin from CORTEX FRAXINI. [Method] Using the content of esculetin measured by HPLC as the index, the optimal process of extracting esculetin was determined by L9(34) orthogonal test. With the isolation of esculetin as the index, the optimum isolation and purification process was determined by polyamide column. [Result] The optimal extraction process was extracting three times, each extraction 1.5 h, with eight-fold amount of 95% ethanol. Polyamide column could effectively isolate esculetin by following the steps below: the test solution was loaded on polyamide column at an adsorption flow rate of 8 BV/h and eluted with distilled water at an elution flow rate of 4 BV/h. The volume ratio of loading solution to resin was set at 8∶ 1 (g/ml) (W/V). Under the above conditions, the total elution rate and refining rate of esculetin were more than 90% and 50%, respectively. [Conclusion] The method used in the study is good for extracting esculetin from CORTEX FRAXINI.

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作者(Author): YU Ling;LIU Ying;YAN Ting;WEN Meng;

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