Medicinal Plant

2016, v.7(Z5) 21-23

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Content Determination about Semi-finished Products of Astragalus Saponin
Content Determination about Semi-finished Products of Astragalus Saponin

Siwei CHEN;Fang WANG;Dan ZHANG;

摘要(Abstract):

[Objectives] For qualitative and quantitative investigation of Astragalus saponin semi-finished products,to establish the Astragalus content determination method,determine the content of Astragalus saponins in semi-finished products,and provide full guarantee for the followup preparation and product quality control.[Methods]With Astragaloside A as reference substance,TLC method was used for qualitative determination of the main components in semi-finished products of Astragalus saponin. Using colorimetry,the content was determined,and with sulfuric acid as chromogenic reagent,the wavelength was measured to be 320 nm.[Results]When the concentration of Astragaloside A was 1.04-8.32 μg·ml~(-1),there was a linear correlation between absorbance and concentration,and precision,stability and reproducibility were in line with standards. The content of Astragaloside A in semi-finished products of Astragalus saponin was measured to be 50- 60%. [Conclusions]The sulfuric acid chromogenic method could be used to determine the content of Astragaloside A in semi-finished products of Astragalus saponins,and it had good precision,stability and reproducibility.

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作者(Author): Siwei CHEN;Fang WANG;Dan ZHANG;

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