Medicinal Plant

2016, v.7(Z2) 33-36

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Construction of a Multi-functional T-DNA Insertion Vector
Construction of a Multi-functional T-DNA Insertion Vector

Linsu ZHANG;Meizhen WANG;Shanfa LU;

摘要(Abstract):

[Objective] Plant mutants have been widely used in gene function analysis. T-DNA insertion is a promising approach for mutant library construction. This study aimed to construct a multifunctional T-DNA insertion vector for generating mutant libraries of Salvia miltiorrhiza Bunge.[Methods] By using pBI121 as a starting vector,an intron sequence from S. miltiorrhiza was introduced. Four enhancers were directionally connected by digestion with Ban I restriction endonuclease. GUS gene promoter was removed and GUS gene was moved forward to construct the recombinant vector pBI-INTRON-GUS-4E.[Results] The constructed recombinant vector pBI-INTRON-GUS-4E harbors an intron sequence from S.miltiorrhiza,four enhancers,GUS gene and NPTII gene,which exhibits multiple functions such as activation tagging,gene(promoter) trapping and insertional mutagenesis.[Conclusions]The successfully constructed multifunctional T-DNA insertion vector provided the basis for generating mutant libraries of S.miltiorrhiza and other plant species.

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基金项目(Foundation): Supported by Research Fund for the Doctoral Program of Higher Education of China(20111106110033)

作者(Author): Linsu ZHANG;Meizhen WANG;Shanfa LU;

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