Medicinal Plant

2012, v.3(11) 81-83+86

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Simultaneous Determination of Eight Lignans in FRUCTUS SCHISANDRAE CHINENSIS from Different Habitats by HPLC
Simultaneous Determination of Eight Lignans in FRUCTUS SCHISANDRAE CHINENSIS from Different Habitats by HPLC

HU Jun-yang;LU Tu-lin;MAO Chun-qin;SU Tao;CAI Bao-chang;

摘要(Abstract):

[Objective] To determine the schisandrin, gomisin J, schisandrol B, gomisin G, schisantherin A, deoxyschisandrin, schisandrin B and schisandrin C of FRUCTUS SCHISANDRAE CHINENSIS from different habitats, including Liaoning Province, Hebei Province and Heilongjiang Province, and to supply a detection means for quality control of FRUCTUS SCHISANDRAE CHINENSIS. [Method] Methanolic extract of FRUCTUS SCHISANDRAE CHINENSIS was separated on the chromatographic column Elite ODS-C18 column (250 mm×4. 6 mm, 5 μm) with gradient mobile phase of acetonitrile (A)-water ( B) (50% of A at 0-17 min; 50%-55% of A at 17-25 min; 55%-75% of A at 25-30 min; 75% of A at 30-35 min; 75%-65% of A at 35-40 min; 65%-50% of A at 40-45 min) at the flow rate of 1.0 ml/min. The column temperature was maintained at 30 ℃ and the detection wavelength was set at 217 nm.[Result] Excellent chromatographic separation was achieved for eight studied lignans with good linearity (r>0.999 5) over the studied concentration ranges. The average recovery rates (RSD) (n = 9 ) were 99.75% (0.38%) for schisandrin, 100. 69% (2.31%) for gomisin J, 100.46% (1.39%) for schisandrol B, 99.87% (1.15%) for gomisin G, 100.22% (1.45%) for schisantherin A, 100.15% (0.99%) for deoxyschisandrin, 100.61% (0.25%) for schisandrin B and 101.31% (1.03%) for schisandrin C, respectively. [Conclusion] The HPLC method developed for simultaneous determination of eight lignans is simple and feasible, which can be used for simultaneous quantitative analysis of lignans in FRUCTUS SCHISANDRAE CHINENSIS.

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基金项目(Foundation): Supported by National Science and Technology Major Special Project( 2009ZX09308-004);; Major Project of Education Department of Jiangsu Province ( 09KJA360001);; Acupuncture Key Laboratory Subject of Jiangsu Province ( KJA200906)

作者(Author): HU Jun-yang;LU Tu-lin;MAO Chun-qin;SU Tao;CAI Bao-chang;

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