Medicinal Plant

2016, v.7(Z2) 48-54

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Determination of Absorbed Component in Rat Plasma in Periploca forrestti Schltr. by High Performance Liquid Chromatography
Determination of Absorbed Component in Rat Plasma in Periploca forrestti Schltr. by High Performance Liquid Chromatography

Sushan LU;Huaguo CHEN;Chao ZHAO;Xin ZHOU;Yanfang YAN;Enming HU;

摘要(Abstract):

[Objective] To determine absorbed component in rat plasma in Periploca forrestti Schltr. [Methods] Ultra-performance liquid chromatography-mass spectrometry analysis technology was used. And L_9(3~4) orthogonal test and chlorogenic acid content determination method was applied. Range analysis and ANOVA analysis were carried out. [Results] Periploca forrestti Schltr. was an active component which could be absorbed into the rat blood. Through the range analysis and ANOVA analysis,the optimal extract conditions were obtained. Then,Periploca forrestti Schltr. was analyzed and chlorogenic acid was successfully separated by C18 reversed phase column( 4. 6 mm × 250 mm,5 μm) at wavelength of 327 nm and temperature of 30℃. With the mobile phase consisted of acetonitrile( A) and water( 0. 5% formic acid,B) in the volume ratio of 9∶ 91( v / v) and flow rate of 1. 0 m L / min,peaks showed good shape and separation. The linear equation of chlorogenic acid was y = 223. 8900 x- 0. 225 8,R~2= 0. 999 7 in the range of 8 μg / m L to 192 μg / m and the average recovery was 98. 569% which indicated the method was fine. [Conclusion]The discovery and the established analysis method of chlorogenic acid in Periploca forrestti Schltr. are expected to control the quality of this medicine better.

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基金项目(Foundation): Supported by National Natural Science Foundation of China(81360641);; Guizhou Provincial Research and Development of Modernization and Technology Industry of TCMs([2013]3014);; Guizhou Province Development and Reform Commission of High-tech Projects([(2013)2069]);; Characteristic Key Laboratory of Guizhou Province Education Department(KY[2012]005);; Guizhou Province High Level Creative Talents Cultivation([2015]4033)

作者(Author): Sushan LU;Huaguo CHEN;Chao ZHAO;Xin ZHOU;Yanfang YAN;Enming HU;

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