Medicinal Plant

2011, v.2(07) 18-20

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Deproteinization Methods from Polysaccharides of Armillariella tabescens
Deproteinization Methods from Polysaccharides of Armillariella tabescens

摘要(Abstract):

[Objective] The aim was to obtain the optimum method to remove protein from polysaccharides of Armillariella tabescens.[Method] Deproteinization from the polysaccharides of A.tabescens was conducted by Sevag method,enzyme method,trichloroacetic acid(TCA)method;and the combination of enzyme method and Sevag method;and the optimum deproteinization method was selected.[Result] The combination of enzyme method and Sevag method was better than others with the contents of protein being only 0.18%;the optimum condition for enzyme method was 0.5% enzyme dosage,40 ℃,water bath for 0.5 h and pH=6.[Conclusion] The combination of enzyme method and Sevag method was the optimum method for deproteinization.

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