Medicinal Plant

2014, v.5(02) 57-58+64

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Determination of Rutin and Quercetin Content in Yantai Bupleurum by HPLC
Determination of Rutin and Quercetin Content in Yantai Bupleurum by HPLC

Haixia CHEN;Wenjun LIU;

摘要(Abstract):

[Objective]To determine the content of rutin and quercetin in the whole plant of Yantai Bupleurum by HPLC. [Methods] An Agilent C18column( 250 mm × 4. 6 mm,5 μm) was applied with a mobile phase of acetonitrile- 0. 1% phosphoric acid solution,gradient elution was selected with the flow rate of 1. 0 mL / min,the UV detector was set at 360 nm. [Results]The average recovery rates of rutin and quercetin were 100. 47% and 99. 50%,the RSD were 1. 34% and 0. 41%. The average content of rutin and quercetin in the whole plant of Yantai Bupleurum reached 83. 00 and 102. 68 μg / g. [Conclusions]The method is fast,accurate and reliable. It is suitable for content determination of rutin and quercetin in the whole plant of Yantai Bupleurum.

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基金项目(Foundation): The Development of Science and Technology Project of Traditional Chinese Medicine in Shandong(2011-202)

作者(Author): Haixia CHEN;Wenjun LIU;

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