Medicinal Plant

2013, v.4(Z1) 31-34

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Optimization by Refluxing Extraction of Total Flavonoids from the Leaves of Rubus coreanus Maq. var. corweanus
Optimization by Refluxing Extraction of Total Flavonoids from the Leaves of Rubus coreanus Maq. var. corweanus

Huamei JIANG;Xiangqian WANG;Chunmei SU;Jingjing ZHANG;Junqiang ZHANG;Guishu ZHANG;

摘要(Abstract):

[Objective]This paper explains screening the optimal condition for extracting total flavonoids from the leaves of Rubus coreanus. [Methods]With the yield of total flavonoids content set to be the indicator,4 factors,concentration of extraction agent,solid-liquid ratio,refluxing temperature and extraction duration,were selected by means of orthogonal array design in order to carry out L 9( 34) orthogonal array design. And the content of total flavonoids was determined by spectrophotometry. [Results] The conditions of optimum extraction process were 70%- concentration ethyl alcohol,1∶ 10( g / mL) solid-liquid ratio,80 ℃ of refluxing temperature,and 3 h of extraction duration. Under these conditions,the content of total flavonoids extracted from Rubus coreanus leaves could be 44. 931 3 mg / g,far more than 202. 79 mg /100 g that was reported in the previous documents. [Conclusions] It was verified by the experiment that total flavonoids extracted from of Rubus coreanus leaves by this process was of good stability and reproductibility and had a high rate of sample recycling and certain feasibility.

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基金项目(Foundation): Supported by Mutual Fund Project of Sichuan Department of Science and Technology and Guiyang College(LKG[2013]13);; Key Project of Teaching Contents and Curriculum System Reform of Guizhou Province Education Department in 2013([2013]446);; Guiyang College Teaching Quality and Teaching Reform Project of Guizhou Province(201212180002)

作者(Author): Huamei JIANG;Xiangqian WANG;Chunmei SU;Jingjing ZHANG;Junqiang ZHANG;Guishu ZHANG;

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