Medicinal Plant

2014, v.5(05) 9-12+17

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The Protective Effect of EtOAc Extract from Gastrodia elata on Oxidative Damage of PC_(12) Cells
The Protective Effect of EtOAc Extract from Gastrodia elata on Oxidative Damage of PC_(12) Cells

Chunni HAN;Hanwen YAN;Jinlong WEN;Qing LIN;Xiaohua DUAN;

摘要(Abstract):

[Objective] The purpose of this study was to investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of PC12cells induced by H2O2. [Methods]PC12cells were cultured and exposed to H2O2to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification,colorimetric MTT assay,leakage rate of LDH,intracellular ROS level and T-SOD activity. [Results]Compared with the model group,EtOAc extract from Gastrodia elata could improve the cells morphology significantly,increase the survival rate of 14. 7%(P < 0. 01),decrease the ROS level(P < 0. 01) and the release of LDH(P <0.01) from cells into culture medium,raise intracellular T-SOD activity(P <0.01). [Conclusions]EtOAc extract from Gastrodia elata had obviously antioxidant effect in vitro.

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基金项目(Foundation): National Natural Science Foundation of China(81160514);; Scientific Research Fund Project of Yunnan Education Department(00370120019)

作者(Author): Chunni HAN;Hanwen YAN;Jinlong WEN;Qing LIN;Xiaohua DUAN;

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