Medicinal Plant

2018, v.9(04) 73-76

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Extraction Process and Content Determination of Caffeic Acid in Laggera alata from Different Production Areas of Guangxi
Extraction Process and Content Determination of Caffeic Acid in Laggera alata from Different Production Areas of Guangxi

Jiangcun WEI;Yong CHEN;Zujie QIN;Jiabao MA;Shengbin CHEN;Zuliang QUE;Jinzhou LI;Zijun CHEN;

摘要(Abstract):

[Objectives] To establish a method for determining the content of Laggera alata( D. Don) Sch. Bip. Ex Oliv. using caffeic acid the target component,and to compare the content of caffeic acid in the medicinal materials of L. alata in different production areas of Guangxi.[Methods]The content was determined by Inertsil~ODS-3 chromatographic column C_(18)( 4. 60 mm × 250 mm,5 μm,mobile phase: acetonitrile-0. 1% phosphoric acid( 22∶ 78),detection wavelength: 320 nm,flow rate: 1. 0 m L/min,column temperature: 30℃,and injection volume: 10 μL. [Results] The caffeic acid showed a good linear relationship in the range of injection volume of 0. 025 92-0. 259 2 μg( R =0. 999 5). The average recovery rate was 98. 33%( RSD = 1. 85%). L. alata in different production areas of Guangxi contained the caffeic acid,and there was a great difference in the caffeic acid. L. alata in Baise had the highest content of caffeic acid,while that in Guilin had the lowest content of caffeic acid. [Conclusions]This method can accurately determine the content of caffeic acid and is expected provide a scientific basis for the development and utilization of herbal medicine L. alata.

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基金项目(Foundation): Supported by Project of National Natural Science Foundation(81660701&81260673);; Project of Guangxi Graduate Education Innovation(YJS201625);; Natural Science Foundation Project of Guangxi(2016GXNSFAA380148&2014GXNSFAA118208);; Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6);; Laboratory of Chemistry and Quality Analysis in the Third Level Laboratory for Research of TCM(Zhuang)of State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa[200]No.21)

作者(Author): Jiangcun WEI;Yong CHEN;Zujie QIN;Jiabao MA;Shengbin CHEN;Zuliang QUE;Jinzhou LI;Zijun CHEN;

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