Medicinal Plant

2011, v.2(06) 45-46

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Determination of the Total Flavonoids Extracts of RADIX PUERARIAE by HPLC
Determination of the Total Flavonoids Extracts of RADIX PUERARIAE by HPLC

摘要(Abstract):

[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.

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基金项目(Foundation): Supported by the Science and Technology Project of Zhongshan City(20092A212)

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