Medicinal Plant

2013, v.4(09) 22-24

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Extraction Technology of Polysaccharides from the Leaves of Coix lacryma-jobi L. by Cellulase Method
Extraction Technology of Polysaccharides from the Leaves of Coix lacryma-jobi L. by Cellulase Method

Tingqi PAN;Quantai WEN;Huafang XIAO;Cuixiao LAN;Zudi YAN;Suoyi HUANG;

摘要(Abstract):

[Objective]To optimize the extraction technology of polysaccharides from the leaves of Coix lacryma-jobi L.by cellulase method.[Methods]Effects of pH value,cellulase dosage,extraction time and hydrolysis temperature on the extraction rate of polysaccharides were researched by single factor test and orthogonal test.[Results]The optimal extraction technology of polysaccharides in C.lacryma-jobi leaves was as follows:was 5 pH,50℃hydrolysis temperature,2.5 h extraction time,and 500U cellulase in 1 g powder of C.lacryma-jobi leaves.Under this extraction technology,the average extraction rate of polysaccharides was 14.45%and RSD was 0.18%.[Conclusions]Extracting the polysaccharides from C.lacryma-jobi leaves by cellulase method had the advantages of time saving and high efficiency.

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基金项目(Foundation): Supported by the National Natural Science Foundation of China(No.81360684);; the Guangxi Natural Sciences Found Project(2011GXNSFA018046);; the Special Found Project of Guangxi Traditional Chinese Medical Science and Technology(GZKZ 10-128);; the Project of Guangxi Education Department(201106LX425);; the Project of Guangxi Administration of Traditional Chinese Medicine(gzzc1047);; the Special Project of Open Found of Guangxi Medical Experimental Research Center(KFJJ2011-04);; College Student Innovation Training Project of Guangxi Zhuang Autonomous Region of China([2012]90);; the Founding Project of Ethnic Medicine Association of Youjiang Medical University for Nationalities(20110715)

作者(Author): Tingqi PAN;Quantai WEN;Huafang XIAO;Cuixiao LAN;Zudi YAN;Suoyi HUANG;

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