Medicinal Plant

2010, v.1(11) 18-20

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Optimization of Proliferation Culture Medium of Atractylodes lancea(Thunb.) DC. subsp. luotianensis S. L. Hu et X. F. Feng
Optimization of Proliferation Culture Medium of Atractylodes lancea(Thunb.) DC. subsp. luotianensis S. L. Hu et X. F. Feng

摘要(Abstract):

[Objective] The research was aimed at identifying the optimum culture medium for the proliferation culture of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng,so as to provide a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.[Method] The optimum basic culture medium was screened out by employing tender branch as explants,and the proportion of 6-BA,KT,NAA and IBA was optimized by L9(34) orthogonal test.[Result] The optimum basic culture medium was N68,and the optimum combination of plant growth regulators was 1.0 mg/L 6-BA,1.0 mg/L KT,0.5 mg/L NAA and 0.2 mg/L IBA.[Conclusion] The optimum medium for the proliferation culture was obtained in this study,which provided a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.

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基金项目(Foundation): Supported by Fund Project of Science and Technology Department of Guangxi Province of China (Guangxi Bureau 0322024-3B)

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