Medicinal Plant

2012, v.3(04) 33-35

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Study on the Liquid Suspension Culture of Dendrobium candidum Wall.ex Lindl. Seeds
Study on the Liquid Suspension Culture of Dendrobium candidum Wall.ex Lindl. Seeds

摘要(Abstract):

[Objective] To study the liquid suspension culture of Dendrobium candidum Wall.ex Lindl. seeds. [Method] 15 formulas of culture liquids were designed with 5 influencing factors for the liquid suspension culture of D. candidum seeds, including MS basal medium, hormone, natural additives, pH and sucrose. Of these, the optimal condition for the liquid suspension culture of D. candidum seeds was screened. [Result] Moderate salt concentration, NAA, low pH value, higher sucrose concentration and moderate potato juice were conducive to the germination of D. candidum seeds. The culture medium suitable for seeds germination was 1/2 MS + 0.5 mg/L NAA + 80 g/L potato juice + 45 g/L sucrose. [Conclusion] The medium formula suitable for liquid suspension culture was selected, which provided theoretical basis for the cultivation of D. candidum.

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