Medicinal Plant

2013, v.4(09) 25-28+31

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Dynamic Changes in Several Physiological Indices of Viola yedoensis Makino under Abiotic Stress
Dynamic Changes in Several Physiological Indices of Viola yedoensis Makino under Abiotic Stress

Chun ZHANG;Ye ZHU;Ying HE;Yuanchun ZHUANG;


[Objective]To determine the dynamic changes in several physiological indices of Viola yedoensis Makino under salinity,cadmium(Cd)stress and drought stresses,so as to deducing its physiological mechanism and laying foundation for further investigating the molecular mechanism and functional genes in response to abiotic stresses.[Method]Employing both hydroponic culture and potted cultivation methods,different salt concentration,Cd concentration and soil water content were set up on the plants with similar growth vigor for measuring MDA content,POD and SOD activities at various growth periods.[Results]Stressed by 0.4%Na2SO4,180μmol/L Cd and 12%of soil moisture,the physiological indices investigated showed regular change trends,indicating the graded response process of V.yedoensis to environmental factors.The activities of protective enzymes were the highest at 54 h,30 h and 3 d after salinity,Cd and drought stress respectively,when the expression of regulatory genes may peak in response to stress.[Conclusion]Whether MDA can be used as a physiological index for evaluating drought and salinity resistance of V.yedoensis still needs further experimental evidence,while the synergistic effect of protective enzymes POD and SOD can prevent cell membrane of V.yedoensis from damage caused by Cd and drought stresses.




基金项目(Foundation): Supported by National Natural Science Foundation of China(81001700);; Sichuan Provincial Department of Education(11ZB124);; Program for Application and Basic Research from Sichuan Provincial Department of Science and Technology(2012JY0081)

作者(Author): Chun ZHANG;Ye ZHU;Ying HE;Yuanchun ZHUANG;