Medicinal Plant

2019, v.10(06) 30-31+51

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Determination of Naringin Content in Peel of Guangxi Citrus maxima (Burm.) Merr by HPLC
Determination of Naringin Content in Peel of Guangxi Citrus maxima (Burm.) Merr by HPLC

Chenyan LIANG;Lu ZHANG;Cuiliu PAN;Yunli TANG;Rongling MA;

摘要(Abstract):

[Objectives] To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.) Merr. [Methods] The high performance liquid chromatography(HPLC) method was applied. The chromatographic column was Agilent HC-C_(18)(4.6 mm×250 mm, 5 μm); the mobile phase was acetonitrile-0.1% phosphoric acid(18∶82); the flow rate was 1.0 mL/min; the column temperature was 25 ℃; the detection wavelength was 283 nm. [Results] Naringin showed a good linear relationship in the range of 0.164-3.27 μg, r=0.999 9. The average recovery rate was 98.66%, and RSD=1.80%(n=6). [Conclusions] This method is simple, feasible, reproducible, and accurate, so it can be used for the determination of naringin content in the peel of Guangxi C. maxima.

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基金项目(Foundation): Supported by Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0324);; Program of Collaborative Innovation Center of Zhuang and Yao Medicine(zyx[2015]-01);; Project of Guangxi University of Chinese Medicine(P2010016)

作者(Author): Chenyan LIANG;Lu ZHANG;Cuiliu PAN;Yunli TANG;Rongling MA;

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