Medicinal Plant

2010, v.1(08) 54-56

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Determination of Resveratrol and the Other Three Constituents of Polygonum Cusprdatum Sieb.et Zucc by HPLC
Determination of Resveratrol and the Other Three Constituents of Polygonum Cusprdatum Sieb.et Zucc by HPLC

摘要(Abstract):

[Objective] To establish a method for simultaneous determination of resveratrol,polydatin,emodin and physcion in Giant Knotweed Rhizome by HPLC.[Methods] The HPLC method was established with DIKMA C18 column(200 mm×4.6 mm,5 μm)and a mobile phase of acetonitrile-0.1% phosphoric acid.The flow rate was 1.0 ml/min;the detection wavelength was 303 nm and the column temperature was 30 ℃.[Results]the linear regression equation of resveratrol,polydatin,emodin and physcion were y = 7 116 169x + 15 631(r=0.999 9),y= 395 313x + 50(r=0.999 9),y=2 752 869x-266 4(r=0.999 9) and y = 463 866x-1 413(r=0.999 9),and present good linear relationship within 0.026-0.520 μg,0.045-0.765 μg,0.026-0.520 μg and 0.026-0.442 μg,respectively.[Conclusion]The method is feasible with good repeatability,and can be used for quality control of Giant Knotweed Rhizome.

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