Medicinal Plant

2019, v.10(06) 77-86

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Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells
Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells

Wangyuan ZENG;Wenxin DAI;

摘要(Abstract):

[Objectives] To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells. [Methods] The in vitro cytology studies were carried out to confirm the expression of the AURKB gene in HCT 116 cells and make clear its role in cell activity, cell cycle control and apoptosis, and investigate the effect of AURKB gene in colorectal cancer(CRC). Quantitative reverse transcription/polymerase chain reaction(PCR) analysis and immunofluorescence(IF) staining for markers of AURKB gene were used to examine the effect of AURKB on HCT 116. The AURKB gene target was examined using western blot analysis. In addition, inhibition of AURKB expression was examined using RNA interference(RNAi) on HCT 116 cells in vitro. [Results] HCT 116 cells infected with AURKB shRNA virus suppressed expression of AURKB in vitro. AURKB gene knockdown HCT 116 cells showed reducing cell apoptosis in vitro. Finally, it demonstrated that AURKB function can induce apoptosis of HCT cells. [Conclusions] AURKB is a key regulator of colorectal cancer. AURKs are potential novel molecular targets for the prevention of cancer cell proliferation.

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作者(Author): Wangyuan ZENG;Wenxin DAI;

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