Medicinal Plant

2016, v.7(Z4) 37-38

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Microscopic and TLC Identification on Herbal Slice of Chinese Honeylocust Abnormal Fruit
Microscopic and TLC Identification on Herbal Slice of Chinese Honeylocust Abnormal Fruit

Wei ZHAO;Yijun CHEN;Yatao HAN;Yuan LIU;

摘要(Abstract):

[Objective] To establish the microscopic and TLC method for the herbal slice of Chinese Honeylocust Abnormal Fruit in Standard on the Chinese Herbal Slice Preparation in Sichuan Province( 2015). [Methods] Conventional powder microscopic identification and TLC.[Results]The microscopic characteristics could be seen about exocarp cells,fibers,clusters of calcium oxalate,lignified parenchyma cells,polycrystalline fibers,calcium oxalate crystals and pitted vessels; the TLC conditions took chloroform-methanol-2 water-glacial acetic acid( 18∶ 1∶ 0. 6∶ 0. 2),toluene-ethyl acetate-formic acid( 14∶ 4∶ 0. 5),and chloroform-ethyl acetate-methanol-water( 15∶ 40∶ 22∶ 10) as developing agents,respectively,and 10% sulfuric acid and ethanol solution as chromogenic agent; the Chinese Honeylocust Abnormal Fruit sample and the control herbs shared the consistent corresponding spots,the color was clear,and the separation effect was good. [Conclusions]The microscopic characteristics of Chinese Honeylocust Abnormal Fruit powder showed strong specificity; the three kinds of TLC conditions had strong specificity controllability,easy operation and high accuracy,which could quickly identify Chinese Honeylocust Abnormal Fruit herbs.

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Abstract:

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基金项目(Foundation): Supported by the 13th Five-Year Plan of National Key Technology Program of China(2015BAC05B02);; Standard on the Chinese Herbal Slice Preparation in Sichuan Province of Sichuan Provincial Food and Drug Inspection Institute(2014)

作者(Author): Wei ZHAO;Yijun CHEN;Yatao HAN;Yuan LIU;

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