Medicinal Plant

2013, v.4(09) 32-34

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Extraction and Identification of Ursolic Acid in HERBA VERBENAE
Extraction and Identification of Ursolic Acid in HERBA VERBENAE

Kaiquan LI;Manping XIONG;

摘要(Abstract):

[Objective]To research the preparation technology of ursolic acid in HERBA VERBENAE,and to establish its content detection method.[Methods]Ursolic acid was extracted and isolated from HERBA VERBENAE by ethanol extraction and agglutination separation.And content detection was established by the method of HPLC.[Results]After the extraction and isolation,the sample was further identified by spectroscopy,and was verified to be ursolic acid.The purity of ursolic acid reached 99.8%.When detecting its content by HPLC,ursolic acid showed good linear relationship within the range of 4.6-23.0μg/mL(r=0.999 9);and the average recovery rate was 99.82%(RSD was0.75%).[Conclusions]This preparation technology was advanced,rational,practical,feasible,and could be applied in the industrialized production.Detecting the content of ursolic acid by HPLC method was accurate and reliable.

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基金项目(Foundation): Supported by the National "863" Plan Project of China[2002AA2Z3217]

作者(Author): Kaiquan LI;Manping XIONG;

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