Medicinal Plant

2010, v.1(10) 38-40

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Separation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC
Separation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC

摘要(Abstract):

[Objective] To separate and prepare gentiopicroside and sweroside from the extracts of Swertia franchetiana H. Smith by high speed counter-current chromatography (HSCCC). [Method] Chloroform∶ methanol∶ water=4∶4∶2 (V/V/V) as solvent system, the upper one was stationary phase while the lower one was mobile phase; ultrasonic degassed for 20 min; the rotating velocity of 850 r/min; mobile phase flow rate of 2 ml/min; the detective wavelength of 254 nm; the purity of product was identified by polarimeter. [Result] 14 mg gentiopricroside and 27 mg swersoside were obtained from 100 mg crude extracts of Swertia franchetiana H. Smith, the specific rotation [a]tD of which were -196.3° and -126.6°respectively, which suggested the products were of high purity. [Conclusion] The study would lay a feasible way for the large scale preparation of gentiopicroside and sweroside by HSCCC.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation):

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享