Medicinal Plant

2013, v.4(Z1) 1-4+7

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Study on Inhibiting Effect of Ursolic Acid on Growth and Apoptosis of Human Hepatocarcinoma Cell Line SMMC-7721
Study on Inhibiting Effect of Ursolic Acid on Growth and Apoptosis of Human Hepatocarcinoma Cell Line SMMC-7721

Lifang CHENG;Xia XU;Bingbing CUI;Hong CHENG;Lijing HOU;Qing YANG;Jin LIU;Yanling MU;Xuesheng YAN;Keming LI;

摘要(Abstract):

[Objective]To explore the inhibiting effect of ursolic acid on human hepatocarcinoma cell line SMMC-7721. [Methods]Human hepatocarcinoma cell line SMMC-7721 was co-cultured with ursolic acid of different concentrations. After cultured for 24 and 72 h,the absorbancy was determined by neutral red stain. And with it,we could evaluate the inhibiting effect of ursolic acid on the proliferation of hepatoma cell line. The effect of ursolic acid on cell apoptosis and cell cycle of hepatoma carcinoma cell was determined by flow cytometry. [Results] The inhibiting effect of ursolic acid on the proliferation of SMMC-7721 cell was significant( P < 0. 05 or P < 0. 01) and it was time dependent and dose-dependent. The median inhibitory concentrations( IC 50) of 24 and 72 h were 12.59 and 8.32 mg/L. The detection result of flow cytometry indicated that apoptosis rate of ursolic acid on human hepatocarcinoma cell line SMMC-7721 reached 14. 07%. And the apoptosis rate rose with the increase of drug concentration. Ursolic acid could block the S phase of SMMC-7721 cell and its effect was positively correlated with dose. [Conclusions] Ursolic acid could significantly inhibit the proliferation of human hepatocarcinoma cell line SMMC-7721. And the antitumor effect of which might be correlated with its effect of inducing cell apoptosis and blocking cell cycle to S phase.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): National Special Project of New Medicine Development(2010ZX09401);; Shangdong Science and Technology Key Project(2011GSF11902)

作者(Author): Lifang CHENG;Xia XU;Bingbing CUI;Hong CHENG;Lijing HOU;Qing YANG;Jin LIU;Yanling MU;Xuesheng YAN;Keming LI;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享