Medicinal Plant

2010, v.1(08) 79-81

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HPLC-ELSD Fingerprint of Bulbus Fritillariea unibractea from Different Producing Areas
HPLC-ELSD Fingerprint of Bulbus Fritillariea unibractea from Different Producing Areas

摘要(Abstract):

[Objective]To establish a HPLC-ELSD fingerprint analysis method of Fritillaria unibractea,and provide references for the quality control of Fritillaria unibracteata.[Methods]The separation was performed on an Agilent Extend-C18(4.6 mm × 250 mm,5 μm)with gradient mobile phase consisting of water(0.02% Triethylamine) and carbinol.The flow rate was 1.0 ml/min.The temperature of drift tube was 90 ℃ and the volume flow of carrier gas was 2.3 L/min.The sampling volume was 10 μl and the temperature of column was 25 ℃.[Results]The fingerprint chromatogram of 16 batches of Fritillaria unibractea from different producing areas was set up,with 15 common peaks shown.[Conclusion]The method was accuracy and can be used in the identification and quality control of Fritillaria unibractea.

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基金项目(Foundation): Supported by Eleventh Five-Year Supporting Project of Ministry of Science and Technology of China(2006BAI09B05-3);; Special Fund for Scientific Research of Central Research Institutions for Public Interest (NO.1381);; Special Fund in the Pharmaceutical Industry (200707007,200807020,200707042);; Special Fund for the Development of New Important Medicine(2009ZX09502-025)

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