Medicinal Plant

2011, v.2(09) 43-45

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Extraction and Determination of Emodin in Polygonum perfoliatum L.
Extraction and Determination of Emodin in Polygonum perfoliatum L.

摘要(Abstract):

[Objective] To extract and determine the content of emodin in Polygonum perfoliatum L.[Method] The content of emodin was determined by reverse phase HPLC.A column of Kromasil C18 was used and a mixture of methanol-water-glacial acetic acid(87∶13∶215) was used as the mobile phase at a flow rate of 110 ml/min.The UV detection wavelength was 254 nm and the column temperature was room temperature.[Result] The emodin showed a good linear relationship within the range of 1-40 μg/ml(r=0.999 9) ;the linear regression equation was Y=112.89X-8.912(r=0.999 9) .The optimum extraction technology was 80% ethanol concentration,35 ml ethanol dosage and 2 h extraction time.The average recovery rate was 99.16%(RSD=1.49%) .[Conclusion] The method was simple,rapid and accurate.It could be used for the extraction and content determination of emodin from Polygonum perfoliatum L.

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基金项目(Foundation): Supported by the Science and Technology Project of Traditional Chinese Medicine by Guangxi Administration of Traditional Chinese Medicine(GZ-KZ09-52);; the Project of Education Department of Guangxi

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