Medicinal Plant

2012, v.3(10) 78-80

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Comparison of the Effective Components Extraction from Compound Chinese Herbal Medicine by Two Different Methods
Comparison of the Effective Components Extraction from Compound Chinese Herbal Medicine by Two Different Methods

XIE Shu-ling;ZHANG Li;

摘要(Abstract):

[Objective] To compare the effective components extraction from compound Chinese herbal medicine by microwave-assisted extraction and ethanol reflux extraction. [Method] Effective components were extracted from compound Chinese herbal medicine by ethanol reflux extraction and microwave-assisted extraction. At the same time, baicalin and puerarin contents were detected by UV-Vis spectrophotometry. With the extraction rates of baicalin and puerarin as the indices, extraction efficiency of the two extraction methods were compared. And the effects of extraction time on extraction rate by microwave-assisted extraction were studied. [Result] Ethanol with different concentrations was used for reflux extraction. Extraction rates of baicalin and puerarin firstly enhanced and then reduced as the ethanol concentration increased. When using microwave-assisted extraction, extraction rates of baicalin and puerarin were the highest by 60% ethanol. When the ethanol concentration was 60%, extraction rates of baicalin and puerarin by microwave-assisted extraction were significantly higher than those by ethanol reflux extraction. The optimal extraction time of microwave-assisted extraction was 15min. Under this condition, the extraction rates of baicalin and puerarin were the highest, which were 0.3155 and 0.5025mg/g, respectively. [Conclusion] Microwave-assisted ethanol reflux extraction could significantly enhance the extraction rates of baicalin and puerarin.

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作者(Author): XIE Shu-ling;ZHANG Li;

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