Medicinal Plant

2010, v.1(07) 56-58

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Isolation of Genomic DNA in Morinda citrifolia Linn.and Establishment of Its ISSR-PCR Reaction System
Isolation of Genomic DNA in Morinda citrifolia Linn.and Establishment of Its ISSR-PCR Reaction System

摘要(Abstract):

[Objective] The research aimed to isolate the genomic DNA in Morinda citrifolia Linn.and establish its ISSR-PCR reaction system.[Method] The Morinda citrifolia Linn.leaves of 3 germplasm resources from Hainan and 4 germplasm resources from America were chosen as test materials,the isolation of DNA in Morinda citrifolia Linn.and establishment of ISSR-PCR were studied.[Result] The results showed that DNA of Morinda citrifolia Linn.leaves with high quality were gained by the modified CTAB method.The isolated DNA could be used for ISSR analysis and 7 primers were found to amplify polymorphic products.[Conclusion]The rapid and efficient extraction method and the ISSR analysis procedure in this experiment have laid a good foundation for application of ISSR in Morinda citrifolia Linn.genetic research.

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基金项目(Foundation): Supported by "948" Project of State Forestry Administration,P. R.China (2007-4-08);; Key Foundation of Landscape Plants and Ornamental Horticulture of Yunnan Province (500974)

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