Medicinal Plant

2015, v.6(Z1) 38-40

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Isolation,Identification and Determination of Verbascoside in Callicarpa longissima(Hemsl.) Merr
Isolation,Identification and Determination of Verbascoside in Callicarpa longissima( Hemsl. ) Merr

Buming LIU;Wei GAO;Mingsheng CHEN;Yan HUANG;

摘要(Abstract):

[Objective] To research on the isolation,identification and determination of verbascoside in Callicarpa longissima( Hemsl.)Merr. [Methods]Verbascoside was extracted by 95% ethanol,isolated and purified by column chromatography and PHPLC from the stem and branch of Callicarpa longissima( Hemsl.) Merr. A HPLC method was set up to determine the content of verbascoside in Callicarpa longissima( Hemsl.) Merr,using ECOSIL ODS- EXTEND column( 4. 6 mm × 250 mm,5 μm),and acetonitrile-0. 2%- phosphoric acid water solution as the mobile phase,the UV detection wavelength was at 334 nm,with the flow rate of 1. 0 m L / min at room temperature.[Results] A good linear was obtained in the range of 0. 051 9 μg- 0. 829 6 μg and the average recovery was 99. 13%( RSD = 1. 80%,n = 9).[Conclusions]Verbascoside was isolated from the plant for the first time. This detection method was simple,feasible and repeatable,and was reliable for the quality control of C. longissima.

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基金项目(Foundation): Supported by the Project of Biological Pharmaceutical Industry and Scientific Instruments by Sharing Service Platform Construction Equipment in Nanning of Guangxi(20121052-3)

作者(Author): Buming LIU;Wei GAO;Mingsheng CHEN;Yan HUANG;

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