Medicinal Plant

2012, v.3(02) 23-25+29

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Trace Analysis on Aristolochic acid A in RADIX ET RHIZOMA ASARI from Different Production Areas
Trace Analysis on Aristolochic acid A in RADIX ET RHIZOMA ASARI from Different Production Areas

摘要(Abstract):

[Objective] To determine and compare the content of aristolochic acid A in RADIX ET RHIZOMA ASARI from 12 different production areas by establishing Reversed-Phase High Performance Liquid Chromatography(RP-HPLC).[Method] The content of aristolochic acid A in the extract of RADIX ET RHIZOMA ASARI prepared by ultrasonic extraction was determined by RP-HPLC.RP-HPLC used the chromatographic column Zorbax SB-C18(4.6 mm ×150 mm,5 μm) with the detection wavelength at 316 nm.Following the injection of 5 μl of sample solution at room temperature,an elution with acetonitrile-water solution(V/V,50:50) as mobile phase was initiated at a flow rate of 1.0 ml/min.[Result] Except the RADIX ET RHIZOMA ASARI in Fengcheng,no aristolochic acid A was detected.The content of aristolochic acid A in RADIX ET RHIZOMA ASARI produced in Fengcheng showed good linear relationship within the range of 2.04 to 20.4 ng/ml.[Conclusion] This method is rapid and simple with high recovery rate and good linear relationship,sensitivity and repeatability.Thus,it could be used for the determination of aristolochic acid A content in RADIX ET RHIZOMA ASARI.And the RADIX ET RHIZOMA ASARI used in this research had relatively high quality.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by the Science and Technology Research Project of Liaoning Provincial Education Department (2009A382)

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享