Medicinal Plant

2014, v.5(02) 51-53+56

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Contents of Forsythin and Forsythiaside A in Different Parts of Forsythia suspensa and Its Medicinal Value
Contents of Forsythin and Forsythiaside A in Different Parts of Forsythia suspensa and Its Medicinal Value

Jinming WANG;Shengci FAN;Anping LI;Tingting CHEN;Bin SUN;Xia YANG;

摘要(Abstract):

[Objective]To compare the contents of forsythin and forsythiaside A in different parts of Forsythia suspensa( Thunb.) Vahl.,and to provide scientific references for the development and utilization of F. suspensa resources. [Methods] Contents of forsythin in the fruits, leaves,branches and fruit stems of F. suspensa were detected by HPLC. Chromatographic column was Luna5uC18( 2) 100A( 250 mm ×4.6 mm, 5 μm). As for forsythin,the mobile phase was acetonitrile- water( 25∶ 75,V / V); detection wavelength was 277 nm; column temperature was room temperature and flow rate was 1. 0 mL / min. As for forsythiaside A,the mobile phase was acetonitrile- 0. 4% glacial acetic acid solution( 15∶85,V/V); detection wavelength was 330 nm; column temperature was room temperature and flow rate was 1.0 mL/min. [Results]Forsythin had good linear relationship within 3. 33- 13. 32 μg / mL( R2= 0. 999 7); the average recovery rate was 99. 30% and the RSD was 1. 4%. Forsythiaside A had good linear relationship within 4. 17- 6. 69 μg / mL( R2= 0. 999 7); the average recovery rate was 98. 26% and the RSD was 1. 8%. [Conclusions]Forsythin content was the maximum in leaves,followed with fruits and branches; and its content was the minimum in fruit stems. Forsythiaside A content was also the maximum in leaves,followed with fruits and fruit stems; and branches had the minimum content.

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基金项目(Foundation): Supported by the Scientific and Technological Support Project during the"12th Five-Year Plan"(2011BA107B01);; Key Science and Technology Program of Shanxi Province(20120313015-9);; the Science and Technology Planning Project of Changzhi City(20118033-1)

作者(Author): Jinming WANG;Shengci FAN;Anping LI;Tingting CHEN;Bin SUN;Xia YANG;

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