Medicinal Plant

2014, v.5(10) 16-20

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HPLC-QTOFMS Analysis of the Chemical Compounds in CORTEX MOUTAN
HPLC-QTOFMS Analysis of the Chemical Compounds in CORTEX MOUTAN

Wenjun ZHAO;Yang LIN;Pengfei LI;Yang LIU;

摘要(Abstract):

[Objective]To effectively identify the main compounds of CORTEX MOUTAN by HPLC-QTOFMS technology. [Methods]CAPCELL PAK C18column( 3. 0 mm × 100 mm,3 μm) was adopted. The mobile phase was 0. 1% methanoic acid solution-acetonitrile with gradient elution; column temperature was 25 ℃; injection volume was 2 μL; and flow rate was 0. 5 mL / min. Time of flight mass spectrometry was used for mass spectrum qualitative detection; and positive and negative ions modes were used for scanning. [Results]A total of 48 compounds were identified from CORTEX MOUTAN by HPLC-QTOFMS technology under the optimized conditions. [Conclusions]This method was rapid,accurate and sensitive,and could be used for the quality control of CORTEX MOUTAN.

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作者(Author): Wenjun ZHAO;Yang LIN;Pengfei LI;Yang LIU;

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