Medicinal Plant

2012, v.3(02) 35-37

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Determination of Resveratrol Content in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC
Determination of Resveratrol Content in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC

摘要(Abstract):

[Objective] Based on optimizing a variety of experimental conditions,a method for the determination of resveratrol in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC was established by using ethanol as extractant.[Method] Phenomenex C18(250 mm×4.6 mm,5 μm) was selected as column,with methanol-0.25% acetic acid(V/V,65:35) as mobile phase,0.8 ml/min mobile speed and 306 nm detection wavelength.Taking the extraction rate of resveratrol as the evaluation index,single factor test and L25(54) orthogonal test were used to optimize the experimental conditions.[Result] The extraction conditions for resveratrol were 60% ethanol,1:15(g/ml) solid-liquid ratio,55 ℃ extraction temperature and 15 min extraction time.Under these conditions,the extraction rate of resveratrol reached 0.826 μg/g.[Conclusion] This method is proved to be simple,rapid,accurate and could be used for the determination of resveratrol content in RHIZOMA ET RADIX POLYGONI CUSPIDATI.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by the Project of State Ethnic Affairs Commission(09GZ07);; Key Project of Education Department of Guizhou Province(2007051)

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享