Medicinal Plant

2010, v.1(10) 31-32

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Determination of Linarin in RADIX et RHIZOMA JATAMANSI VALERIANAE by RP-HPLC
Determination of Linarin in RADIX et RHIZOMA JATAMANSI VALERIANAE by RP-HPLC

摘要(Abstract):

[Objective] To determine the content of linarin in RADIX et RHIZOMA JATAMANSI VALERIANAE by RP-HPLC method.[Method] The HPLC conditions were as follows:Zorbax SB C18 column as separation column(150 mm×4.6 mm,5 μm),mobile phase of methanol-0.1% acetic acid(V:V=70:30),flow rate of 1.0 ml/min,column temperature of 30 ℃ and detective wavelength of 326 nm.[Result] The linear relationship between linarin concentration and peak area was good in the range of 0.010-0.080 μg,(r=0.999 7).The average recovery rate of linarin was 98.2% with RSD value of 1.78%(n=5).[Conclusion] The RP-HPLC method established in this study is favorable for the determination of linarin in Valeriana wallichii DC.,which would provide reference for the quality control of Valeriana wallichii DC..

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基金项目(Foundation): Supported by the Project of Jiangsu Animal Husbandry and Veterinary College (YB201001)

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