Medicinal Plant

2013, v.4(04) 17-19

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Changes of the Ellagic Acid Content in the Granules of PERICARPIUM GRANATI Extracts with Different Storage Periods
Changes of the Ellagic Acid Content in the Granules of PERICARPIUM GRANATI Extracts with Different Storage Periods

Jie REN;Jingming YAO;Guotao WU;Zhenhua FAN;Juan ZHAO;Xin WU;Wenjun LIU;Dongxia MENG;

摘要(Abstract):

[Objective]To discuss the changes of ellagic acid content in the granules of PERICARPIUM GRANATI extracts with different storage periods,and to detect the stability of the granules.[Methods]High performance liquid chromatography was adopted.Chromatographic column was PT-230AColume heater Apollo C 18 column (150mm×4.6mm,5μm); column temperature was 30℃ ; mobile phase was aceto-nitrile-1.2% phosphoric acid solution (20∶80,V/V); detection wavelength was 254 nm and flow rate was 1.0 mL/min.[Results]The lin-ear range of the detection of ellagic acid content was between 0.52-5.20 μg/mL(R2 = 0.997 9); the average recovery rate was 97.8%; and RSD was 1.6%.The content of ellagic acids in the granules of PERICARPIUM GRANATI extracts was 0.495 mg/g on March 25,2008,and that was 0.439 mg/g on March 25,2011,which indicated that the granules of PERICARPIUM GRANATI extracts had good stability,and the period of validity could reach more than 3 years.[Conclusions]This method was simple and accurate and could be used for the quality control of PERICARPIUM GRANATI.

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基金项目(Foundation): Supported by the Overseas Returnee Research Fund of Shanxi Province([2002]16-92)

作者(Author): Jie REN;Jingming YAO;Guotao WU;Zhenhua FAN;Juan ZHAO;Xin WU;Wenjun LIU;Dongxia MENG;

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