Medicinal Plant

2010, v.1(10) 10-11+15

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

Determination of Ursolic Acid and Oleanolic Acid in the Root of Agastache rugosa by RP-HPLC
Determination of Ursolic Acid and Oleanolic Acid in the Root of Agastache rugosa by RP-HPLC

摘要(Abstract):

[Objective] To establish a quantitative method for simultaneous determination of ursolic acid and oleanolic acid in the roots of Agastache rugosa by RP-HPLC, so as to provide basis for the development of Agastache rugosa. [Method] C18 chromatographic column (4.6 mm×250 mm, 5 μm), mobile phase of acetonitrile-methanol-0.3% H3PO4 (V ∶V∶V=60∶30∶10), flow rate of 0.8 ml/min, detective wavelength of 210 nm, room temperature. [Result]The good linear relationship was obtained under the above conditions. The recovery rates of the ursolic acid and oleanolic acid were 99.6% and 98.9% with RSD of 3.12% and 1.99% respectively. The average contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa were 0.138 mg/g and 0.147 mg/g respectively. [Conclusion]The method is rapid, precise and reliable, which can be used to determine the contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa. This case would offer theoretical evidence for developing medicinal value of Agastache rugosa.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation): Supported by Eleventh Five-Year Plan of Science and Technology of Jilin Provincial Education Department (2009269)

作者(Author):

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享